Journal:

Article Title: Salmonella enterica Serovar Typhimurium bla PER-1 -Carrying Plasmid pSTI1 Encodes an Extended-Spectrum Aminoglycoside 6?- N -Acetyltransferase of Type Ib

doi: 10.1128/AAC.47.2.697-703.2003

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: Strains were grown in Luria-Bertani (LB) broth or LB agar, which was supplemented, when required, with ampicillin (100 μg/ml) or kanamycin (50 μg/ml). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Phenotype, genotype, or relevant characteristics a Source or reference Strains Escherichia coli BHM 71-18 mutS thi supE Δ( lac-proAB ) [ mutS ::Tn 10 ] [ F ′ proAB laqI q Z Δ M15 ] Promega E. coli JM109 endA1 recA1 gyrA96 thi hsdR17 ( r K − m K + ) relA1 supE44 Δ( lac-proAB )[ F ′ traD36 proAB laq1 q Z Δ M15 ] Promega E. coli DH5α deoR endA1 gyrA96 hsdR17 ( r K − m K + ) recA1 relA1 supE44 thi-1 Δ( lacZYA-argFV169 ) φ80 lacZ Δ M15 F − Clontech Citrobacter freundii CFr564 (pLMM564) Net r Tob r Gen r [AAC(6′)-Ib 7 (Gln118, Ser119)] Str r Spc r Sul r 4 Enterobacter cloacae EC1563(pLMM563) Net r Tob r Gen r [AAC(6′)-Ib 8 (Gln118, Ser119)] Str r Spc r Sul r Cmp r Tet r 4 Salmonella serovar Typhimurium(pSTI1) Net r Tob r Gen r [AAC(6′)-Ib 11 (Leu118, Ser119)] Str r Spc r Sul r 35 Plasmids pAZ505 pBR322 containing a 1.5-kb fragment [AAC(6′)-Ib (Gln118, Leu119)] Amp r Net r Tob r Ami r 34 pGEM Amp r , cloning vector Promega pGEMM564 1.06-bp Eae I fragment [AAC(6′)-Ib 7 ] of pLMM564 cloned into pGEM 11 pGEMM563 1.09-bp Eae I fragment [AAC(6′)-Ib 8 ] of pLMM563 cloned into pGEM This study pGEMMST 1.14-bp Eae I fragment [AAC(6′)-Ib 11 ] of pST11 cloned into pGEM This study pGEMM564M Derivative of pGEMM564 [AAC(6′)-Ib 7 (Gln118 replaced by Leu)] This study pGEMM563M Derivative of pGEMM563 [AAC(6′)-Ib 8 (Gln118 replaced by Leu)] This study pGEMMSTM Derivative of pGEMMST [AAC(6′)-Ib 11 (Leu118 replaced by Gln)] This study pGEMMSTP1 Derivative of pGEMMST [AAC(6′)-Ib 11 (A325TG replaced by AGG)] This study pGEMMSTP2 Derivative of pGEMMST [AAC(6′)-Ib 11 (A325TG replaced by GGG)] This study Open in a separate window a Ami, amikacin; Amp, ampicillin; Cmp, chloramphenicol; Gen, gentamicin; Net, netilmicin; Spc, spectinomycin; Str, streptomycin; Sul, sulfonamide; Tet, tetracycline; Tob, tobramycin.

Techniques: Plasmid Preparation, Cloning, Clone Assay

Journal:

Article Title: Salmonella enterica Serovar Typhimurium bla PER-1 -Carrying Plasmid pSTI1 Encodes an Extended-Spectrum Aminoglycoside 6?- N -Acetyltransferase of Type Ib

doi: 10.1128/AAC.47.2.697-703.2003

Figure Lengend Snippet: Resistance profiles of AAC(6′)-Ib 7 , AAC(6′)-Ib 8 , and AAC(6′)-Ib 11 and correlation between resistance phenotypes and amino acids at positions 118 and 119 a

Article Snippet: Strains were grown in Luria-Bertani (LB) broth or LB agar, which was supplemented, when required, with ampicillin (100 μg/ml) or kanamycin (50 μg/ml). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Phenotype, genotype, or relevant characteristics a Source or reference Strains Escherichia coli BHM 71-18 mutS thi supE Δ( lac-proAB ) [ mutS ::Tn 10 ] [ F ′ proAB laqI q Z Δ M15 ] Promega E. coli JM109 endA1 recA1 gyrA96 thi hsdR17 ( r K − m K + ) relA1 supE44 Δ( lac-proAB )[ F ′ traD36 proAB laq1 q Z Δ M15 ] Promega E. coli DH5α deoR endA1 gyrA96 hsdR17 ( r K − m K + ) recA1 relA1 supE44 thi-1 Δ( lacZYA-argFV169 ) φ80 lacZ Δ M15 F − Clontech Citrobacter freundii CFr564 (pLMM564) Net r Tob r Gen r [AAC(6′)-Ib 7 (Gln118, Ser119)] Str r Spc r Sul r 4 Enterobacter cloacae EC1563(pLMM563) Net r Tob r Gen r [AAC(6′)-Ib 8 (Gln118, Ser119)] Str r Spc r Sul r Cmp r Tet r 4 Salmonella serovar Typhimurium(pSTI1) Net r Tob r Gen r [AAC(6′)-Ib 11 (Leu118, Ser119)] Str r Spc r Sul r 35 Plasmids pAZ505 pBR322 containing a 1.5-kb fragment [AAC(6′)-Ib (Gln118, Leu119)] Amp r Net r Tob r Ami r 34 pGEM Amp r , cloning vector Promega pGEMM564 1.06-bp Eae I fragment [AAC(6′)-Ib 7 ] of pLMM564 cloned into pGEM 11 pGEMM563 1.09-bp Eae I fragment [AAC(6′)-Ib 8 ] of pLMM563 cloned into pGEM This study pGEMMST 1.14-bp Eae I fragment [AAC(6′)-Ib 11 ] of pST11 cloned into pGEM This study pGEMM564M Derivative of pGEMM564 [AAC(6′)-Ib 7 (Gln118 replaced by Leu)] This study pGEMM563M Derivative of pGEMM563 [AAC(6′)-Ib 8 (Gln118 replaced by Leu)] This study pGEMMSTM Derivative of pGEMMST [AAC(6′)-Ib 11 (Leu118 replaced by Gln)] This study pGEMMSTP1 Derivative of pGEMMST [AAC(6′)-Ib 11 (A325TG replaced by AGG)] This study pGEMMSTP2 Derivative of pGEMMST [AAC(6′)-Ib 11 (A325TG replaced by GGG)] This study Open in a separate window a Ami, amikacin; Amp, ampicillin; Cmp, chloramphenicol; Gen, gentamicin; Net, netilmicin; Spc, spectinomycin; Str, streptomycin; Sul, sulfonamide; Tet, tetracycline; Tob, tobramycin.

Techniques: Plasmid Preparation

Journal: mBio

Article Title: Emergence of the Novel Aminoglycoside Acetyltransferase Variant aac(6′)-Ib-D179Y and Acquisition of Colistin Heteroresistance in Carbapenem-Resistant Klebsiella pneumoniae Due to a Disrupting Mutation in the DNA Repair Enzyme MutS

doi: 10.1128/mBio.01954-20

Figure Lengend Snippet: Comparison of of qnrB91 and qnrB2 fluoroquinolone MICs for K. pneumoniae ATCC 13883 ^a

Article Snippet: ATCC 13883/pSTV aac(6′)-Ib-W102R , Arg , WT , 0.06 , 0.06 , 0.25 , 0.01 , 0.5 (×>2) , 2 (×4).

Techniques: Comparison

Journal: mBio

Article Title: Emergence of the Novel Aminoglycoside Acetyltransferase Variant aac(6′)-Ib-D179Y and Acquisition of Colistin Heteroresistance in Carbapenem-Resistant Klebsiella pneumoniae Due to a Disrupting Mutation in the DNA Repair Enzyme MutS

doi: 10.1128/mBio.01954-20

Figure Lengend Snippet: Comparison of fluoroquinolone and aminoglycoside MICs of aac(6′)-Ib-D179Y and other aac(6′)-Ib variants in K. pneumoniae ATCC 13883 ^a

Article Snippet: ATCC 13883/pSTV aac(6′)-Ib-W102R , Arg , WT , 0.06 , 0.06 , 0.25 , 0.01 , 0.5 (×>2) , 2 (×4).

Techniques: Comparison

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Identification and Characterization of Conjugative Plasmids That Encode Ciprofloxacin Resistance in Salmonella

doi: 10.1128/AAC.00575-18

Figure Lengend Snippet: Genetic and phenotypic characteristics of ciprofloxacin-resistant Salmonella strains and the corresponding transconjugants

Article Snippet: In addition to the ciprofloxacin resistance phenotype, the conjugative plasmids also carried resistance to ampicillin, tetracycline, and streptomycin ( ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain ID a Species, parental strain, or serotype PMQR genes Approx plasmid size (kb) MIC b AMK CTX LEV CIP CIP + Ihi KAN OLA STR CRO TET CHL NAL AMP MRP SXT ATCC 25922 E. coli 4 0.06 0.015 0.0075 0.0075 2 16 8 0.03 2 4 1 8 0.015 1 J53 (Azi r ) E. coli ≤0.5 ≤0.015 0.015 0.015 0.0075 ≤0.5 4 2 ≤0.015 0.5 1 2 2 ≤0.125 4 Sa63 S . London qnrB6 – aac(6′)-Ib-cr 113 4 0.12 0.5 2 1 16 16 >128 0.06 >32 >64 16 >64 0.06 >32 TC-Sa63 E. coli J53 qnrB6 – aac(6′)-Ib-cr 113 2 0.06 0.5 2 0.5 16 16 128 0.03 32 2 8 >64 0.12 16 Sa81 Salmonella enterica serotype O3,O10 qnrB6 – aac(6′)-Ib-cr 113 4 0.12 0.5 2 1 16 32 >128 0.06 >32 >64 16 >64 0.12 32 TC-Sa81 E. coli J53 qnrB6 – aac(6′)-Ib-cr 113 1 0.06 0.25 1 0.5 4 8 128 0.03 >32 2 8 >64 0.12 16 Sa100 S . Thompson qnrB6 – aac(6′)-Ib-cr 113 4 2 1 4 1 32 64 >128 0.25 >32 >64 32 >64 0.12 >32 TC-Sa100 E. coli J53 qnrB6 – aac(6′)-Ib-cr 113 2 0.25 0.25 2 0.5 16 16 128 0.06 32 2 8 >64 0.12 32 Sa76 S . London qnrB6 – aac(6′)-Ib-cr 104 4 0.12 0.5 2 1 16 16 >128 0.06 >32 >64 16 >64 0.06 32 TC-Sa76 E. coli J53 qnrB6 – aac(6′)-Ib-cr 104 1 0.06 0.25 1 0.5 4 16 64 0.06 >32 2 8 >64 0.12 16 Sa117 S . London qnrB6 – aac(6′)-Ib-cr 104 4 0.12 0.5 1 1 16 32 >128 0.06 >32 >64 16 >64 0.12 32 TC-Sa117 E. coli J53 qnrB6 – aac(6′)-Ib-cr 104 1 0.06 0.25 1 0.5 4 8 128 ≤0.015 16 2 8 >64 0.12 16 Sa4 S . Typhimurium qnrS1 237 2 0.12 0.5 1 0.5 2 32 16 0.06 1 >64 8 >64 0.12 >32 TC-Sa4 E. coli J53 qnrS1 237 ≤0.5 0.06 0.5 0.5 0.5 ≤0.5 8 8 0.06 0.5 2 8 >64 0.06 16 Sa48 S . London 1 0.06 0.12 0.015 0.0075 8 16 >128 0.06 >32 64 8 16 0.06 8 Open in a separate window a ID, identification; TC, transconjugant. b AMK, amikacin; CTX, cefotaxime; LEV, levofloxacin; CIP, ciprofloxacin; Ihi, Phe-Arg-β-naphthylamide (80 μg/ml); KAN, kanamycin; OLA, olaquindox; STR, streptomycin; CRO, ceftriaxone; TET, tetracycline; CHL, chloramphenicol; NAL, nalidixic acid; AMP, ampicillin; MRP, meropenem; SXT, trimethoprim-sulfamethoxazole.

Techniques: Plasmid Preparation

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Identification and Characterization of Conjugative Plasmids That Encode Ciprofloxacin Resistance in Salmonella

doi: 10.1128/AAC.00575-18

Figure Lengend Snippet: Alignment of ciprofloxacin-resistant conjugative IncFIB(K)-type plasmids from Salmonella using the BLAST Ring Image Generator (BRIG). Plasmids pSa76-CIP (104,666 bp), pSa63-CIP (112,943 bp), and pCVM19633_110 (accession number CP001125) in the NCBI database were analyzed. The sequence of pSa63-CIP was used as a reference (red circle), and key genetic loci are labeled. Plasmid sequences were generated through the combination of Illumina and PacBio sequencing data. The aac(6′)-Ib-cr gene is annotated as aac(6′)-Ib.

Article Snippet: In addition to the ciprofloxacin resistance phenotype, the conjugative plasmids also carried resistance to ampicillin, tetracycline, and streptomycin ( ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain ID a Species, parental strain, or serotype PMQR genes Approx plasmid size (kb) MIC b AMK CTX LEV CIP CIP + Ihi KAN OLA STR CRO TET CHL NAL AMP MRP SXT ATCC 25922 E. coli 4 0.06 0.015 0.0075 0.0075 2 16 8 0.03 2 4 1 8 0.015 1 J53 (Azi r ) E. coli ≤0.5 ≤0.015 0.015 0.015 0.0075 ≤0.5 4 2 ≤0.015 0.5 1 2 2 ≤0.125 4 Sa63 S . London qnrB6 – aac(6′)-Ib-cr 113 4 0.12 0.5 2 1 16 16 >128 0.06 >32 >64 16 >64 0.06 >32 TC-Sa63 E. coli J53 qnrB6 – aac(6′)-Ib-cr 113 2 0.06 0.5 2 0.5 16 16 128 0.03 32 2 8 >64 0.12 16 Sa81 Salmonella enterica serotype O3,O10 qnrB6 – aac(6′)-Ib-cr 113 4 0.12 0.5 2 1 16 32 >128 0.06 >32 >64 16 >64 0.12 32 TC-Sa81 E. coli J53 qnrB6 – aac(6′)-Ib-cr 113 1 0.06 0.25 1 0.5 4 8 128 0.03 >32 2 8 >64 0.12 16 Sa100 S . Thompson qnrB6 – aac(6′)-Ib-cr 113 4 2 1 4 1 32 64 >128 0.25 >32 >64 32 >64 0.12 >32 TC-Sa100 E. coli J53 qnrB6 – aac(6′)-Ib-cr 113 2 0.25 0.25 2 0.5 16 16 128 0.06 32 2 8 >64 0.12 32 Sa76 S . London qnrB6 – aac(6′)-Ib-cr 104 4 0.12 0.5 2 1 16 16 >128 0.06 >32 >64 16 >64 0.06 32 TC-Sa76 E. coli J53 qnrB6 – aac(6′)-Ib-cr 104 1 0.06 0.25 1 0.5 4 16 64 0.06 >32 2 8 >64 0.12 16 Sa117 S . London qnrB6 – aac(6′)-Ib-cr 104 4 0.12 0.5 1 1 16 32 >128 0.06 >32 >64 16 >64 0.12 32 TC-Sa117 E. coli J53 qnrB6 – aac(6′)-Ib-cr 104 1 0.06 0.25 1 0.5 4 8 128 ≤0.015 16 2 8 >64 0.12 16 Sa4 S . Typhimurium qnrS1 237 2 0.12 0.5 1 0.5 2 32 16 0.06 1 >64 8 >64 0.12 >32 TC-Sa4 E. coli J53 qnrS1 237 ≤0.5 0.06 0.5 0.5 0.5 ≤0.5 8 8 0.06 0.5 2 8 >64 0.06 16 Sa48 S . London 1 0.06 0.12 0.015 0.0075 8 16 >128 0.06 >32 64 8 16 0.06 8 Open in a separate window a ID, identification; TC, transconjugant. b AMK, amikacin; CTX, cefotaxime; LEV, levofloxacin; CIP, ciprofloxacin; Ihi, Phe-Arg-β-naphthylamide (80 μg/ml); KAN, kanamycin; OLA, olaquindox; STR, streptomycin; CRO, ceftriaxone; TET, tetracycline; CHL, chloramphenicol; NAL, nalidixic acid; AMP, ampicillin; MRP, meropenem; SXT, trimethoprim-sulfamethoxazole.

Techniques: Sequencing, Labeling, Plasmid Preparation, Generated